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FEATURED PUBLICATIONS

 

Intracellular nucleosomes constrain a DNA linking number difference of -1.26 that reconciles the Lk paradox.
Joana Segura, Ricky S. Joshi, Ofelia Díaz-Ingelmo, Antonio Valdés, Silvia Dyson, Belén Martínez-García and Joaquim Roca.
Nature Communications Sep 28;9(1):3989 (2018)


DNA knots occur in intracellular chromatin.
Valdes, A., Segura, J., Dyson, S., Martinez-Garcia, B., and Roca, J.
Nucleic Acids Res 46, 650-660 (2018)

 

Electrophoretic Analysis of the DNA Supercoiling Activity of DNA Gyrase.
Martinez-Garcia, B., Valdes, A., Segura, J., Dyson, S., Diaz-Ingelmo, O., and Roca, J.
Methods Mol Biol 1805, 291-300 (2018)

 

Structure and Chromosomal Organization of Yeast Genes Regulated by Topoisomerase II.
Joshi, R.S., Nikolaou, C., and Roca, J.
Int J Mol Sci 19 (2018).

 

Genome urbanization: clusters of topologically co-regulated genes delineate functional compartments in the genome of Saccharomyces cerevisiae.
Tsochatzidou, M., Malliarou, M., Papanikolaou, N., Roca, J., and Nikolaou, C.
Nucleic Acids Res 45, 5818-5828 (2017)

 

In silico, in vitro and in vivo imageries of type II topoisomerases.
Roca, J.
Phys Life Rev 18, 147-149 (2016)

 

DNA Topology and Global Architecture of Point Centromeres.
Diaz-Ingelmo, O., Martinez-Garcia, B., Segura, J., Valdes, A., and Roca, J.
Cell Rep 13, 667-677 (2015)

 

Topoisomerase II minimizes DNA entanglements by proofreading DNA topology after DNA strand passage
Martinez-Garcia, B., Fernandez, X., Diaz-Ingelmo, O., Rodriguez-Campos, A., Manichanh, C., and Roca, J.
Nucleic Acids Res 42, 1821-1830 (2014)

 

Chromatin regulates DNA torsional energy via topoisomerase II-mediated relaxation of positive supercoils.
Fernandez, X., Diaz-Ingelmo, O., Martinez-Garcia, B., and Roca, J.
Embo J 33, 1492-1501 (2014)

 

Topoisomerase II regulates yeast genes with singular chromatin architectures.
Nikolaou, C., Bermudez, I., Manichanh, C., Garcia-Martinez, J., Guigo, R., Perez-Ortin, J.E., and Roca, J.
Nucleic Acids Res 41, 9243-9256 (2013)

 

Topoisomerase II is required for the production of long Pol II gene transcripts in yeast.
Joshi, R.S., Pina, B., and Roca, J. (2012)
Nucleic Acids Res 40, 7907-7915.

 

Transcriptional inhibition by DNA torsional stress.
Roca, J.
Transcription 2, 82-85 (2011)

 

The torsional state of DNA within the chromosome.
Roca, J.
Chromosoma 120, 323-334 (2011)

 

Positional dependence of transcriptional inhibition by DNA torsional stress in yeast chromosomes.
Joshi, R.S., Pina, B., and Roca, J.
Embo J 29, 740-748 (2010)

 

A method for genome-wide analysis of DNA helical tension by means of psoralen-DNA photobinding.
Bermudez, I., Garcia-Martinez, J., Perez-Ortin, J.E., and Roca, J.
Nucleic Acids Res 38, e182 (2010)

 

Expression of transgenic PPP1CC2 in the testis of Ppp1cc-null mice rescues spermatid viability and spermiation but does not restore normal sperm tail ultrastructure, sperm motility, or fertility.
Soler, D.C., Kadunganattil, S., Ramdas, S., Myers, K., Roca, J., Slaughter, T., Pilder, S.H., and Vijayaraghavan, S.
Biol Reprod 81, 343-352 (2009)

 

Two-dimensional agarose gel electrophoresis of DNA topoisomers.
Roca, J.
Methods Mol Biol 582, 27-37 (2009)

 

Topoisomerase II: a fitted mechanism for the chromatin landscape.
Roca, J.
Nucleic Acids Res 37, 721-730 (2009)

 

Production of highly knotted DNA by means of cosmid circularization inside phage capsids.
Trigueros, S., and Roca, J.
BMC Biotechnol 7, 94 (2007)

 

Fractal dimension and localization of DNA knots.
Ercolini, E., Valle, F., Adamcik, J., Witz, G., Metzler, R., De Los Rios, P., Roca, J., and Dietler, G.
Phys Rev Lett 98, 058102 (2007)

 

Topoisomerase II, not topoisomerase I, is the proficient relaxase of nucleosomal DNA.
Salceda, J., Fernandez, X., and Roca, J.
Embo J 25, 2575-2583 (2006)

 

DNA knots reveal a chiral organization of DNA in phage capsids.
Arsuaga, J., Vazquez, M., McGuirk, P., Trigueros, S., Sumners, D., and Roca, J.
Proc Natl Acad Sci U S A 102, 9165-9169 (2005)

 

Asymmetric removal of supercoils suggests how topoisomerase II simplifies DNA topology.
Trigueros, S., Salceda, J., Bermudez, I., Fernandez, X., and Roca, J.
J Mol Biol 335, 723-731 (2004)

 

The path of the DNA along the dimer interface of topoisomerase II.
Roca, J.
J Biol Chem 279, 25783-25788 (2004)

 

Cloning, functional analysis and post-transcriptional regulation of a type II DNA topoisomerase from Leishmania infantum. A new potential target for anti-parasite drugs.
Hanke, T., Ramiro, M.J., Trigueros, S., Roca, J., and Larraga, V.
Nucleic Acids Res 31, 4917-4928 (2003).

 

A GyrB-GyrA fusion protein expressed in yeast cells is able to remove DNA supercoils but cannot substitute eukaryotic topoisomerase II.
Trigueros, S., and Roca, J.
Genes Cells 7, 249-257 (2002)

 

Failure to relax negative supercoiling of DNA is a primary cause of mitotic hyper-recombination in topoisomerase-deficient yeast cells.
Trigueros, S., and Roca, J.
J Biol Chem 277, 37207-37211 (2002)

 

Knotting probability of DNA molecules confined in restricted volumes: DNA knotting in phage capsids.
Arsuaga, J., Vazquez, M., Trigueros, S., Sumners, D., and Roca, J.
Proc Natl Acad Sci U S A 99, 5373-5377 (2002)

 

Circular minichromosomes become highly recombinogenic in topoisomerase-deficient yeast cells.
Trigueros, S., and Roca, J.
J Biol Chem 276, 2243-2248 (2001)

 

Novel display of knotted DNA molecules by two-dimensional gel electrophoresis.
Trigueros, S., Arsuaga, J., Vazquez, M.E., Sumners, D.W., and Roca, J.
Nucleic Acids Res 29, E67-67. (2001).

 

Filter binding assays for topoisomerase-DNA complexes.
Roca, J.
Methods Mol Biol 95, 75-80 (2001)

 

Varying levels of positive and negative supercoiling differently affect the efficiency with which topoisomerase II catenates and decatenates DNA.
Roca, J.
J Mol Biol 305, 441-450 (2001).

 

Two-dimensional agarose-gel electrophoresis of DNA topoisomers.
Hanai, R., and Roca, J.
Methods Mol Biol 94, 19-27 (1999)

 

The probabilities of supercoil removal and decatenation by yeast DNA topoisomerase II.
Roca, J., and Wang, J.C.
Genes Cells 1, 17-27 (1996)

 

DNA transport by a type II topoisomerase: direct evidence for a two-gate mechanism.
Roca, J., Berger, J.M., Harrison, S.C., and Wang, J.C.
Proc Natl Acad Sci U S A 93, 4057-4062 (1996)

 

The mechanisms of DNA topoisomerases.
Roca, J.
Trends Biochem Sci 20, 156-160 (1995)

 

DNA transport by a type II DNA topoisomerase: evidence in favor of a two-gate mechanism.
Roca, J., and Wang, J.C.
Cell 77, 609-616 (1994).

 

Antitumor bisdioxopiperazines inhibit yeast DNA topoisomerase II by trapping the enzyme in the form of a closed protein clamp.
Roca, J., Ishida, R., Berger, J.M., Andoh, T., and Wang, J.C.
Proc Natl Acad Sci U S A 91, 1781-1785 (1994)

 

On the simultaneous binding of eukaryotic DNA topoisomerase II to a pair of double-stranded DNA helices.
Roca, J., Berger, J.M., and Wang, J.C.
J Biol Chem 268, 14250-14255 (1993)

 

Separation of rooster spermatogenic nuclei by means of centrifugal elutriation.
Boix, J., and Roca, J.
Cytometry 14, 465-467 (1993)

 

The capture of a DNA double helix by an ATP-dependent protein clamp: a key step in DNA transport by type II DNA topoisomerases.
Roca, J., and Wang, J.C.
Cell 71, 833-840 (1992)

 

A hit-and-run system for targeted genetic manipulations in yeast.
Roca, J., Gartenberg, M.R., Oshima, Y., and Wang, J.C.
Nucleic Acids Res 20, 4671-4672 (1992)

 

DNA topoisomerase II activity in nonreplicating, transcriptionally inactive, chicken late spermatids.
Roca, J., and Mezquita, C.
Embo J 8, 1855-1860 (1989).

 

 

 
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